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Enzymatic glutathionylation reactions are tightly controlled and highly specific and are considered a major post-translational modification that occurs in response to fluctuations in local redox environments

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Only when we tested up to 10 mM ophthalmic acid, the reaction velocity was reduced by 1020% (Supplementary Fig

Briefly, flash-frozen tissue samples were homogenized in ice-cold PBS containing 2 mM EDTA (1 ml PBS/EDTA per 10 mg tissue) using a Dounce homogenizer